Durand Adeline, Brown Tom
School of Chemistry, University of Southampton, Highfield, Southampton, UK.
Nucleosides Nucleotides Nucleic Acids. 2007;26(6-7):785-94. doi: 10.1080/15257770701501534.
Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces. To fulfil this requirement on lipid membranes we have synthesised a thymidine monomer modified with a cholesterol moiety. Solution studies show that the melting temperature (Tm) of the duplex, with adjacent cholesterols on each strand, is much higher than that of the unmodified duplex.
高度选择性的碱基对识别使DNA成为有序自组装结构的合适构建块。在纳米技术的某些应用中，DNA复合物需要固定在表面上。为了满足在脂质膜上的这一要求，我们合成了一种用胆固醇部分修饰的胸腺嘧啶单体。溶液研究表明，每条链上带有相邻胆固醇的双链体的解链温度（Tm）比未修饰的双链体高得多。
