Tainaka Kazuki, Fujiwara Yoshimasa, Okamoto Akimitsu
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):155-6. doi: 10.1093/nass/49.1.155.
A solvatochromic dye is very useful in studies of local polarity and dynamics in biological systems. A novel solvatochromic chromophore, Nile Red beta-C-2'-deoxyriboside, has been synthesized to act as a photophysical probe, and incorporated site-selectively into an oligodeoxynucleotide. The absorption and fluorescence spectra of Nile Red nucleoside showed a remarkable sensitivity to the solvent polarity.
一种溶剂化显色染料在生物系统中局部极性和动力学的研究中非常有用。一种新型的溶剂化显色发色团，尼罗红β-C-2'-脱氧核糖苷，已被合成用作光物理探针，并被位点选择性地掺入到寡脱氧核苷酸中。尼罗红核苷的吸收光谱和荧光光谱对溶剂极性表现出显著的敏感性。
