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荧光环状胞嘧啶核苷的合成及其掺入寡核苷酸后的荧光性质。

Synthesis of fluorescent cyclic cytosine nucleosides and their fluorescent properties upon incorporation into oligonucleotides.

作者信息

Mizuta Masahiro, Miyata Kenichi, Seio Kohji, Santa Tomofumi, Sekine Mitsuo

机构信息

Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2006(50):19-20. doi: 10.1093/nass/nrl010.

DOI:10.1093/nass/nrl010
PMID:17150796
Abstract

We report here the synthesis and spectroscopic characterization of a new fluorescent pyrimidopyrimidoindole nucleoside derivative (dC(PPI)) modified at the cytosine base. The photophysical properties of dC(PPI) were examined by fluorescent spectroscopy and quantum chemical calculations. It was found that dC(PPI)-labeled oligonucleotides gave almost the same thermal stability as that of the corresponding unmodified sequences. In addition, the quantum yield of dC(PPI) in the double-stranded state was significantly higher than that in the single-stranded state.

摘要

我们在此报告一种在胞嘧啶碱基处修饰的新型荧光嘧啶并嘧啶吲哚核苷衍生物（dC(PPI)）的合成及光谱表征。通过荧光光谱和量子化学计算研究了dC(PPI)的光物理性质。发现用dC(PPI)标记的寡核苷酸与相应未修饰序列具有几乎相同的热稳定性。此外，dC(PPI)在双链状态下的量子产率显著高于单链状态。

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