Chen Liwei, Yu Xuechun, Wang Dan
Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA.
Ultramicroscopy. 2007 Apr-May;107(4-5):275-80. doi: 10.1016/j.ultramic.2006.06.006. Epub 2006 Jul 14.
We show that inconsistent-imaging dynamics, in which the cantilever oscillates in the attractive regime on substrate background but in the repulsive regime on sample, leads to artifacts in apparent height in AC mode Atomic force microscopy. Active Q control can be used to effectively tune the imaging dynamics. Increased effective Q promotes the attractive regime, improves imaging sensitivity, and results in less invasive imaging of soft biological molecules.
我们表明,在非一致成像动力学中,悬臂在基底背景上处于吸引状态下振荡,但在样品上处于排斥状态下振荡,这会在交流模式原子力显微镜的表观高度中产生伪像。有源Q控制可用于有效调节成像动力学。有效Q的增加促进了吸引状态,提高了成像灵敏度,并实现了对软生物分子的侵入性更小的成像。