Major F, Turcotte M, Gautheret D, Lapalme G, Fillion E, Cedergren R
Département d'Informatique et de Recherche Opérationnelle, Université de Montréal, Québec, Canada.
Science. 1991 Sep 13;253(5025):1255-60. doi: 10.1126/science.1716375.
Three-dimensional (3-D) structural models of RNA are essential for understanding of the cellular roles played by RNA. Such models have been obtained by a technique based on a constraint satisfaction algorithm that allows for the facile incorporation of secondary and other structural information. The program generates 3-D structures of RNA with atomic-level resolution that can be refined by numerical techniques such as energy minimization. The precision of this technique was evaluated by comparing predicted transfer RNA loop and RNA pseudoknot structures with known or consensus structures. The root-mean-square deviation (2.0 to 3.0 angstroms before minimization) between predicted and control structures reveal this system to be an effective method in modeling RNA.
RNA的三维（3-D）结构模型对于理解RNA在细胞中所起的作用至关重要。此类模型是通过一种基于约束满足算法的技术获得的，该算法便于纳入二级结构和其他结构信息。该程序生成具有原子级分辨率的RNA三维结构，可通过能量最小化等数值技术进行优化。通过将预测的转运RNA环和RNA假结结构与已知或一致结构进行比较，评估了该技术的精度。预测结构与对照结构之间的均方根偏差（最小化前为2.0至3.0埃）表明该系统是一种模拟RNA的有效方法。
