Matthews J L, Sogandares-Bernal F, Judy M M, Marengo-Rowe A J, Leveson J E, Skiles H, Newman J T, Chanh T C
Baylor Research Foundation, Southern Methodist University, Dallas, Texas.
Transfusion. 1991 Sep;31(7):636-41. doi: 10.1046/j.1537-2995.1991.31791368342.x.
The transmission of human immunodeficiency virus (HIV) by blood or blood components is a major concern in blood banking. A photodynamic flow cell system was designed to inactivate cell-free HIV mixed with blood from a healthy donor. Blood containing 4 x 10(3) infectious units of HIV was treated with 10 and 20 micrograms per mL of commercially available dihematoporphyrin ether (DHE) per mL. Aliquots of this mixture were then held in the dark or irradiated in a flow cell illuminated at a light energy density of 5 J per cm2 provided by a xenon light source equipped with a 630 +/- 5 nm band-pass interference filter; the aliquots were subsequently placed in A.301 cells. All infected cultures were assessed for reverse transcriptase (RT) activity for 17 days. RT activity for either concentration of dye was significantly reduced in irradiated samples as compared to that in samples held in the dark. Blood samples from volunteers also were assessed for the effects of the inactivation process on red cells at concentrations of DHE up to 200 micrograms per mL. No effects were observed on red cell 2,3 DPG or ATP, whole blood potassium concentrations, red cell osmotic fragility, or blood cell antigens.
通过血液或血液成分传播人类免疫缺陷病毒（HIV）是血库中的一个主要问题。设计了一种光动力流动池系统，用于灭活与健康供体血液混合的无细胞HIV。含有4×10³个感染单位HIV的血液，每毫升用10微克和20微克市售双血卟啉醚（DHE）进行处理。然后将该混合物的等分试样置于黑暗中，或在配备有630±5nm带通干涉滤光片的氙光源提供的光能量密度为5J/cm²的流动池中进行照射；随后将等分试样置于A.301细胞中。对所有感染培养物进行17天的逆转录酶（RT）活性评估。与置于黑暗中的样品相比，照射样品中两种染料浓度下的RT活性均显著降低。还评估了志愿者血液样本在高达200微克/毫升的DHE浓度下灭活过程对红细胞的影响。未观察到对红细胞2,3 - DPG或ATP、全血钾浓度、红细胞渗透脆性或血细胞抗原的影响。
