猿猴免疫缺陷病毒的光动力灭活

Photodynamic inactivation of simian immunodeficiency virus.

作者信息

Chanh T C, Allan J S, Matthews J L, Sogandares-Bernal F, Judy M M, Skiles H, Leveson J, Marengo-Rowe A, Newman J T

机构信息

Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.

出版信息

J Virol Methods. 1989 Oct;26(1):125-31. doi: 10.1016/0166-0934(89)90081-5.

DOI:10.1016/0166-0934(89)90081-5

PMID:2531753

Abstract

A photodynamic flow system employing a dihematoporphyrin ether (DHE) was tested for its ability to inactivate the in vitro infectivity of simian immunodeficiency virus (SICMac) at 630 +/- 5 nm with a light fluence of 5 J/cm2. Cell-free SIVMac was inactivated by photoactivated hematoporphyrin derivative in a dose-dependent fashion. Since SIVMac is closely related to human immunodeficiency virus type 2 (HIV-2) and we have previously reported the successful photodynamic inactivation of HIV-1 in cell-free medium as well as in whole human blood, this technology has the potential for the eradication of transfusion-associated acquired immunodeficiency diseases caused by the above-mentioned retroviruses.

摘要

测试了一种采用二血卟啉醚（DHE）的光动力流动系统，在630±5纳米波长、光通量为5焦耳/平方厘米的条件下，使其灭活猿猴免疫缺陷病毒（SIVMac）体外感染性的能力。游离的SIVMac被光活化的血卟啉衍生物以剂量依赖方式灭活。由于SIVMac与人类免疫缺陷病毒2型（HIV-2）密切相关，并且我们之前已报道在游离培养基以及全血中成功实现了HIV-1的光动力灭活，这项技术有潜力根除由上述逆转录病毒引起的输血相关获得性免疫缺陷疾病。