Caufield P W, Li Y, Dasanayake A, Saxena D
College of Dentistry, New York University, New York, NY 10010, USA.
Caries Res. 2007;41(1):2-8. doi: 10.1159/000096099.
For nearly a century, lactobacilli (LB) in the oral cavity have been generally associated with dental caries. Here, we characterized the LB isolated from the saliva of 6 women with active caries using genetic-based taxonomical identification methods. From each subject, 30 isolates growing on Rogosa medium and presumed to be LB were analyzed. Of the 180 isolates, 176 were further characterized by biotyping, DNA melting points, DNA chromosomal fingerprinting, genotyping, and phylogenetic cluster assessment. We found a total of 30 unique genotypes of LB in the saliva of caries-active women, with each woman harboring between 2 and 8 distinct genotypes. Although Lactobacillus vaginalis, L. fermentum, and L. salivarius were found in 4 of 6 of the subjects, results from other studies using comparable methods show an entirely different array of LB associated with caries. These collective observations lead us to surmise that LB associated with dental caries are likely exogenous and opportunistic colonizers, arising from food or other reservoirs outside the oral cavity.
近一个世纪以来，口腔中的乳酸菌（LB）通常被认为与龋齿有关。在此，我们使用基于基因的分类鉴定方法，对从6名患有活动性龋齿的女性唾液中分离出的乳酸菌进行了特征分析。从每个受试者中，分析了30株在罗戈萨培养基上生长且推测为乳酸菌的分离株。在这180株分离株中，有176株通过生物分型、DNA熔点、DNA染色体指纹图谱、基因分型和系统发育聚类评估进行了进一步特征分析。我们在患有活动性龋齿的女性唾液中总共发现了30种独特的乳酸菌基因型，每名女性携带2至8种不同的基因型。虽然在6名受试者中的4名中发现了阴道乳酸菌、发酵乳杆菌和唾液乳杆菌，但其他使用类似方法的研究结果显示，与龋齿相关的乳酸菌种类完全不同。这些综合观察结果使我们推测，与龋齿相关的乳酸菌可能是外源性的机会性定植菌，源自口腔外的食物或其他来源。
