Rowley A, Castro B, Levy J, Sullivan J, Koup R, Fresco R, Shulman S
Division of Infectious Diseases, Children's Memorial Hospital, Chicago, IL 60614.
Pediatr Res. 1991 May;29(5):417-9. doi: 10.1203/00006450-199105010-00001.
We and others previously reported DNA polymerase activity in culture supernatants of peripheral blood mononuclear cells from patients with acute Kawasaki syndrome (KS). In the present study, we further characterized the previously detected polymerase activity and attempted to confirm its presence in cultured peripheral blood mononuclear cells from additional patients with KS. Characterization experiments indicated that the polymerase activity was typical of a DNA-dependent DNA polymerase rather than viral reverse transcriptase. Peripheral blood mononuclear cell cultures from 17 additional KS patients were negative for reverse transcriptase activity in three laboratories. Our findings do not provide support for a retroviral etiology of KS. Further studies should continue to focus on infectious agents in efforts to elucidate the etiology of KS.
我们和其他研究人员之前报道过，急性川崎综合征（KS）患者外周血单个核细胞培养上清液中存在DNA聚合酶活性。在本研究中，我们进一步对之前检测到的聚合酶活性进行了表征，并试图在另外一些KS患者的培养外周血单个核细胞中确认其存在。表征实验表明，该聚合酶活性是典型的依赖DNA的DNA聚合酶，而非病毒逆转录酶。另外17名KS患者的外周血单个核细胞培养物在三个实验室检测均未发现逆转录酶活性。我们的研究结果不支持KS由逆转录病毒引起这一病因学观点。进一步的研究应继续聚焦于感染因子，以努力阐明KS的病因。
