Erkel G, Anke T, Velten R, Steglich W
LB-Biotechnologie der Universität, Kaiserslautern, Bundesrepublik Deutschland.
Z Naturforsch C J Biosci. 1991 May-Jun;46(5-6):442-50. doi: 10.1515/znc-1991-5-617.
A novel enzyme inhibitor of RNA-directed DNA-polymerases of avian myeloblastosis and murine leukemia virus was isolated from fermentations of an tasmanian Podoscypha species. Its structure was elucidated by spectroscopic methods and oxidative degradation as (E)-4,5-dioxo-2-hexadecenoic acid (1). The enzyme inhibitor, which was named podoscyphic acid, did not inhibit DNA and RNA synthesis in permeabilized L 1210 cells nor did it affect RNA synthesis in isolated nuclei of L 1210 cells. 1 inhibits protein synthesis in whole L 1210 cells and rabbit reticulocyte lysate and shows very weak antimicrobial and cytotoxic properties. The testing of ethyl (E)-4,5-dioxo-2-hexadecenoate (2) and (E)-4-oxo-2-tetradecenoic acid (11) revealed the importance of the free gamma-oxoacrylic acid unit for the biological activities of 1.
从塔斯马尼亚的一种 Podoscypha 属真菌发酵物中分离出一种新型的禽成髓细胞瘤病毒和鼠白血病病毒的 RNA 指导的 DNA 聚合酶抑制剂。通过光谱方法和氧化降解确定其结构为（E）-4,5-二氧代-2-十六碳烯酸（1）。该酶抑制剂被命名为波多西芬酸，它不抑制通透化的 L1210 细胞中的 DNA 和 RNA 合成，也不影响 L1210 细胞分离细胞核中的 RNA 合成。1 抑制整个 L1210 细胞和兔网织红细胞裂解物中的蛋白质合成，并显示出非常弱的抗菌和细胞毒性。对（E）-4,5-二氧代-2-十六碳烯酸乙酯（2）和（E）-4-氧代-2-十四碳烯酸（11）的测试揭示了游离γ-氧代丙烯酸单元对 1 的生物活性的重要性。
