Erkel G, Anke T, Gimenez A, Steglich W
LB Biotechnologie der Universität, Kaiserslautern, Germany.
J Antibiot (Tokyo). 1992 Jan;45(1):29-37. doi: 10.7164/antibiotics.45.29.
A novel inhibitor of RNA-directed DNA-polymerases was isolated from fermentations of Clavicorona pyxidata. Its structure was elucidated by spectroscopic methods. Clavicoronic acid (1) is a noncompetitive inhibitor of avian myeloblastosis virus (Ki 130 microM) and Moloney murine leukemia virus (Ki 68 microM) reverse transcriptases. In permeabilized cells and isolated nucleic DNA- and RNA-synthesis are not affected. Clavicoronic acid markedly inhibits the multiplication of vesicular stomatitis virus in baby hamster kidney cells by interfering with this virus's RNA-directed RNA-polymerase. 1 exhibits no cytotoxic and very weak antimicrobial activities.
从硬皮马勃（Clavicorona pyxidata）发酵物中分离出一种新型RNA指导的DNA聚合酶抑制剂。通过光谱法阐明了其结构。硬皮马勃酸（1）是禽成髓细胞瘤病毒逆转录酶（Ki为130微摩尔）和莫洛尼鼠白血病病毒逆转录酶（Ki为68微摩尔）的非竞争性抑制剂。在透化细胞和分离的核酸中，DNA和RNA的合成不受影响。硬皮马勃酸通过干扰水泡性口炎病毒的RNA指导的RNA聚合酶，显著抑制该病毒在幼仓鼠肾细胞中的增殖。1没有细胞毒性，抗菌活性非常弱。
