Gotta S L, Miller O L, French S L
Department of Biology, University of Virginia, Charlottesville 22901.
J Bacteriol. 1991 Oct;173(20):6647-9. doi: 10.1128/jb.173.20.6647-6649.1991.
The rate of in vivo transcription elongation for Escherichia coli rRNA operons was determined by electron microscopy following addition of rifampin to log-phase cultures. Direct observation of RNA polymerase positions along rRNA operons 30, 40, and 70 s after inhibition of transcription initiation yielded a transcription elongation rate of 42 nucleotides per s.
通过在对数期培养物中添加利福平后进行电子显微镜观察，测定了大肠杆菌rRNA操纵子的体内转录延伸速率。在转录起始受到抑制后30、40和70秒，直接观察RNA聚合酶沿rRNA操纵子的位置，得出转录延伸速率为每秒42个核苷酸。
