在黑腹果蝇中进行错误表达筛选，旨在揭示参与身体部位形成的新因子。
Misexpression screen in Drosophila melanogaster aiming to reveal novel factors involved in formation of body parts.
作者信息
Grieder Nicole C, Charlafti Ilias, Kloter Urs, Jäckle Herbert, Schäfer Ulrich, Gehring Walter J
机构信息
Biozentrum der Universität Basel, Abteilung Zellbiologie, CH-4056 Basel, Switzerland.
出版信息
Genetics. 2007 Apr;175(4):1707-18. doi: 10.1534/genetics.106.064212. Epub 2006 Dec 18.
Abstract
To identify novel factors that lead a fly imaginal disc to adopt its developmental fate, we carried out a modular dominant misexpression screen in imaginal discs. We have identified two factors that appear to change the fate of the respective body structure and appear to lead to the transformation of a body part. In one mutant line, notum tissue, normally derived from wing imaginal tissue, formed close to the site of the sternopleural bristles, which are leg disc derivatives. In the other line, the arista is transformed into a tubular structure, resembling an abnormal leg. We found that ectopic expression of abrupt was responsible for this potential transformation of the arista.
摘要
为了鉴定导致果蝇成虫盘发育为特定命运的新因子，我们在成虫盘中进行了模块化显性错表达筛选。我们鉴定出了两个因子，它们似乎改变了相应身体结构的命运，并似乎导致了身体部位的转变。在一个突变品系中，通常源自翅成虫组织的背板组织，在位于胸侧板刚毛（腿盘衍生物）的部位附近形成。在另一个品系中，触角转变为管状结构，类似于异常的腿。我们发现，abrupt的异位表达导致了触角的这种潜在转变。
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本文引用的文献
1
Establishment of compartments in the developing leg imaginal discs ofDrosophila melanogaster.在黑腹果蝇发育中的腿部成虫盘内隔室的建立。
Wilehm Roux Arch Dev Biol. 1976 Mar;180(1):9-30. doi: 10.1007/BF00848882.
2
Molecular analysis of the dominant homeotic Antennapedia phenotype.触角足显性同源异形表型的分子分析。
EMBO J. 1987 Jan;6(1):201-6. doi: 10.1002/j.1460-2075.1987.tb04739.x.
3
Suppression of Polycomb group proteins by JNK signalling induces transdetermination in Drosophila imaginal discs.JNK信号通路对多梳蛋白家族的抑制诱导果蝇成虫盘的转决定。
Nature. 2005 Nov 10;438(7065):234-7. doi: 10.1038/nature04120.
4
Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification.编码一种与染色质相关的溴相邻同源结构域蛋白的有翼眼在盘状结构特化中的作用。
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15918-23. doi: 10.1073/pnas.0507945102. Epub 2005 Oct 24.
5
Expression of COPI components during development of Drosophila melanogaster.
Gene Expr Patterns. 2005 Dec;6(1):11-21. doi: 10.1016/j.modgep.2005.06.001. Epub 2005 Oct 5.
6
A transient cell cycle shift in Drosophila imaginal disc cells precedes multipotency.果蝇成虫盘细胞中的短暂细胞周期转变先于多能性。
Cell. 2005 Feb 11;120(3):383-93. doi: 10.1016/j.cell.2004.12.008.
7
Systematic gene targeting on the X chromosome of Drosophila melanogaster.对黑腹果蝇X染色体进行系统性基因靶向。
Chromosoma. 2004 Dec;113(6):271-5. doi: 10.1007/s00412-004-0313-5. Epub 2004 Oct 12.
8
Direct integration of Hox and segmentation gene inputs during Drosophila development.果蝇发育过程中Hox基因与体节分化基因输入的直接整合
Nature. 2004 Oct 7;431(7009):653-9. doi: 10.1038/nature02946.
9
Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt.BTB-锌指蛋白abrupt调控果蝇树突形态多样性的发育
Neuron. 2004 Sep 16;43(6):809-22. doi: 10.1016/j.neuron.2004.08.016.
10
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac.一种利用P元件和piggyBac转座子的黑腹果蝇互补转座子工具包。
Nat Genet. 2004 Mar;36(3):283-7. doi: 10.1038/ng1314. Epub 2004 Feb 22.
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