Vanderford Brett J, Snyder Shane A
Southern Nevada Water Authority, 1350 Richard Bunker Avenue, Henderson, Nevada 89015, USA.
Environ Sci Technol. 2006 Dec 1;40(23):7312-20. doi: 10.1021/es0613198.
A method has been developed for the trace analysis of 15 pharmaceuticals, four metabolites of pharmaceuticals, three potential endocrine disruptors, and one personal care product in various waters. The method employs solid-phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC-MS/MS), using electrospray ionization (ESI) in both positive and negative modes. Unlike many previous LC-MS/MS methods, which suffer from matrix suppression, this method uses isotope dilution for each compound to correct for matrix suppression, as well as SPE losses and instrument variability. The method was tested in five matrices, and results indicate that the method is very robust. Matrix spike recoveries for all compounds were between 88 and 106% for wastewater influent, 85 and 108% for wastewater effluent, 72 and 105% for surface water impacted by wastewater, 96 and 113% for surface water, and 91 and 116% for drinking water. The method reporting limits for all compounds were between 0.25 and 1.0 ng/L, based on 500 mL of sample extracted and a final extract volume of 500 microL. Occurrence of the compounds in all five matrices is also reported.
已开发出一种用于痕量分析各种水体中15种药物、4种药物代谢物、3种潜在内分泌干扰物和1种个人护理产品的方法。该方法采用固相萃取（SPE）和液相色谱/串联质谱（LC-MS/MS），在正、负离子模式下均使用电喷雾电离（ESI）。与许多先前受基质抑制影响的LC-MS/MS方法不同，该方法对每种化合物使用同位素稀释来校正基质抑制以及SPE损失和仪器变异性。该方法在五种基质中进行了测试，结果表明该方法非常稳健。所有化合物在进水废水中的基质加标回收率为88%至106%，在出水废水中为85%至108%，在受废水影响的地表水中为72%至105%，在地表水中为96%至113%，在饮用水中为91%至116%。基于500 mL提取的样品和500 μL的最终提取物体积，所有化合物的方法报告限在0.25至1.0 ng/L之间。还报告了所有五种基质中这些化合物的存在情况。
