Pruden Amy, Pei Ruoting, Storteboom Heather, Carlson Kenneth H
Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Environ Sci Technol. 2006 Dec 1;40(23):7445-50. doi: 10.1021/es060413l.
This study explores antibiotic resistance genes (ARGs) as emerging environmental contaminants. The purpose of this study was to investigate the occurrence of ARGs in various environmental compartments in northern Colorado, including Cache La Poudre (Poudre) River sediments, irrigation ditches, dairy lagoons, and the effluents of wastewater recycling and drinking water treatment plants. Additionally, ARG concentrations in the Poudre River sediments were analyzed at three time points at five sites with varying levels of urban/agricultural impact and compared with two previously published time points. It was expected that ARG concentrations would be significantly higher in environments directly impacted by urban/agricultural activity than in pristine and lesser-impacted environments. Polymerase chain reaction (PCR) detection assays were applied to detect the presence/absence of several tetracycline and sulfonamide ARGs. Quantitative real-time PCR was used to further quantify two tetracycline ARGs (tet(W) and tet(O)) and two sulfonamide ARGs (sul(I) and sul(II)). The following trend was observed with respect to ARG concentrations (normalized to eubacterial 16S rRNA genes): dairy lagoon water > irrigation ditch water > urban/agriculturally impacted river sediments (p < 0.0001), except for sul(II), which was absent in ditch water. It was noted that tet(W) and tet(O) were also present in treated drinking water and recycled wastewater, suggesting that these are potential pathways for the spread of ARGs to and from humans. On the basis of this study, there is a need for environmental scientists and engineers to help address the issue of the spread of ARGs in the environment.
本研究将抗生素抗性基因（ARGs）作为新兴的环境污染物进行探索。本研究的目的是调查科罗拉多州北部各环境介质中ARGs的存在情况，包括卡什拉普德雷（普德雷）河沉积物、灌溉沟渠、奶牛场泻湖以及废水回收处理厂和饮用水处理厂的废水。此外，在受城市/农业影响程度不同的五个地点的三个时间点分析了普德雷河沉积物中的ARG浓度，并与之前发表的两个时间点进行了比较。预计受城市/农业活动直接影响的环境中的ARG浓度将显著高于原始环境和受影响较小的环境。应用聚合酶链反应（PCR）检测方法来检测几种四环素和磺胺类ARGs的存在与否。使用定量实时PCR进一步定量两种四环素ARGs（tet(W)和tet(O)）和两种磺胺类ARGs（sul(I)和sul(II)）。关于ARG浓度（以真细菌16S rRNA基因标准化）观察到以下趋势：奶牛场泻湖水>灌溉沟渠水>受城市/农业影响的河流沉积物（p<0.0001），除了sul(II)，其在沟渠水中不存在。值得注意的是，tet(W)和tet(O)也存在于处理后的饮用水和再生废水中，这表明这些是ARGs在人与环境之间传播的潜在途径。基于本研究，环境科学家和工程师需要帮助解决ARGs在环境中传播的问题。
