Department of Natural Resource Management and Geography, The University of Melbourne, Parkville, Victoria 3010, Australia.
J Water Health. 2010 Sep;8(3):521-31. doi: 10.2166/wh.2010.102. Epub 2010 Mar 9.
The purpose of this study was to investigate the occurrence of antibiotic resistance genes (ARGs) in water used for irrigation in the Werribee River Basin, Australia, including river water and reclaimed effluent water (reclaimed water). Samples of reclaimed water, collected over a one-year period, were screened for the occurrence of ARGs using PCR detection assays. The presence of ARGs in the reclaimed water samples were contrasted with that of water samples taken from the Werribee River Basin, collected over the same time period, from five points selected for varying levels of urban and agricultural impact. Of the 54 river water samples collected, 2 (4%), 2 (4%), 0 and 0 were positive for methicillin, sulfonamide, gentamicin and vancomycin-resistant genes, respectively, while 6 of 11 reclaimed water samples were positive for methicillin (9%) and sulfonamide (45%). The presence/absence of ARGs did not appear to correlate with other measured water quality parameters. The low detection of ARGs in river water indicates that, regardless of its poor quality, the river has not yet been severely contaminated with ARGs. The greater prevalence of ARGs in reclaimed water indicates that this important agricultural water source will need to be monitored into the future.
本研究旨在调查澳大利亚 Werribee 河流域灌溉用水（包括河水和再生污水）中抗生素耐药基因（ARGs）的发生情况。采集了一年期间的再生污水样本，使用 PCR 检测方法对 ARGs 的存在情况进行了筛查。将再生污水样本中的 ARGs 存在情况与同一时期从 Werribee 河流域五个具有不同城市和农业影响水平的地点采集的水样进行了对比。在采集的 54 个河水样本中，分别有 2 个（4%）、2 个（4%）、0 个和 0 个样本对甲氧西林、磺胺类药物、庆大霉素和万古霉素耐药基因呈阳性，而在采集的 11 个再生污水样本中有 6 个样本对甲氧西林（9%）和磺胺类药物（45%）呈阳性。ARGs 的存在/缺失似乎与其他测量的水质参数无关。河水样本中 ARGs 的低检出率表明，无论其水质较差，河流尚未受到 ARGs 的严重污染。再生水中 ARGs 的更高检出率表明，这种重要的农业用水源需要在未来进行监测。
