Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Sci Total Environ. 2013 Aug 1;458-460:267-72. doi: 10.1016/j.scitotenv.2013.04.038. Epub 2013 May 7.
The prevalence of antibiotic resistance genes (ARGs) and their relationship with antibiotics in the surface waters of the Huangpu River and the drinking water sources of Shanghai, China, were investigated. 39 ARGs, including four sulfonamide ARGs, 23 tetracycline ARGs, four chloramphenicol ARGs, five β-lactam ARGs and three penicillin ARGs were targeted in this study. Two sulfonamide ARGs (sul I and sul II), eight tetracycline ARGs (tet(A), tet(B), tet(C), tet(G), tet(M), tet(O), tet(W) and tet(X)), and one β-lactam ARG (TEM) were detected to be present in water samples by polymerase chain reaction (PCR), with the detection frequencies ranging from 42.86% to 100%. The average concentrations of the 11 ARGs ranged from 3.66×10(1) copy/mL (tet(B)) to 1.62×10(5) copy/mL (sul II), quantified by quantitative real-time PCR (QPCR). The number of detected ARGs and the ARG concentrations were generally higher in suburban sampling sites than in urban sites. Being in or near suburban rural areas, some raw drinking water sources were observed to have comparatively higher ARG contamination, drawing an urgent attention for the concern of public health. Generally consistent relations were observed between the concentrations of tet genes and the tetracycline levels and between the concentrations of sul genes and the sulfonamide levels.
本研究调查了中国黄浦江地表水和上海饮用水源中抗生素耐药基因（ARGs）的流行情况及其与抗生素的关系。本研究针对 39 种 ARGs，包括 4 种磺胺类 ARGs、23 种四环素类 ARGs、4 种氯霉素类 ARGs、5 种β-内酰胺类 ARGs 和 3 种青霉素类 ARGs。通过聚合酶链反应（PCR）检测到水样中存在两种磺胺类 ARGs（sul I 和 sul II）、八种四环素类 ARGs（tet(A)、tet(B)、tet(C)、tet(G)、tet(M)、tet(O)、tet(W)和 tet(X)）和一种β-内酰胺类 ARG（TEM），检测频率为 42.86%至 100%。11 种 ARGs 的平均浓度范围为 3.66×10(1)拷贝/mL（tet(B)）至 1.62×10(5)拷贝/mL（sul II），通过实时定量 PCR（QPCR）定量。郊区采样点检测到的 ARGs 数量和 ARG 浓度普遍高于市区采样点。一些原水水源位于或靠近郊区农村地区，观察到 ARG 污染相对较高，引起了对公共健康的关注。通常情况下，tet 基因的浓度与四环素水平之间以及 sul 基因的浓度与磺胺类水平之间存在一致的关系。
