Kanesaki Yu, Yamamoto Hiroshi, Paithoonrangsarid Kalyanee, Shoumskaya Maria, Suzuki Iwane, Hayashi Hidenori, Murata Norio
National Institute for Basic Biology, Okazaki 444-8585, Japan.
Plant J. 2007 Jan;49(2):313-24. doi: 10.1111/j.1365-313X.2006.02959.x. Epub 2006 Dec 20.
Oxidative stress caused by reactive oxygen species and, in particular, to hydrogen peroxide (H(2)O(2)) has a major impact on all biological systems, including plants and microorganisms. We investigated the H(2)O(2)-inducible expression of genes in the cyanobacterium Synechocystis sp. PCC 6803 using genome-wide DNA microarrays. Our systematic screening of a library of mutant lines with defects in histidine kinases (Hiks) by RNA slot-blot hybridization and DNA-microarray analysis suggested that four Hiks, namely, Hik33, Hik34, Hik16 and Hik41, are involved in the perception and transduction of H(2)O(2) signals that regulate the gene expression of 26 of the 77 H(2)O(2)-inducible genes with induction factors higher than 4.0. Among the four Hiks, Hik33 was the main contributor and was responsible for 22 of the 26 H(2)O(2)-inducible genes under the control of the Hiks. By contrast to Hik33, PerR encoding putative peroxide-sensing protein is involved in the regulation of only nine H(2)O(2)-inducible genes.
由活性氧物种，特别是过氧化氢（H₂O₂）引起的氧化应激对包括植物和微生物在内的所有生物系统都有重大影响。我们使用全基因组DNA微阵列研究了集胞藻属PCC 6803中基因的H₂O₂诱导表达。我们通过RNA斑点杂交和DNA微阵列分析对组氨酸激酶（Hiks）存在缺陷的突变体系文库进行系统筛选，结果表明，四个Hiks，即Hik33、Hik34、Hik16和Hik41，参与了H₂O₂信号的感知和转导，这些信号调节了77个H₂O₂诱导基因中26个基因的表达，其诱导因子高于4.0。在这四个Hiks中，Hik33是主要贡献者，负责在Hiks控制下的26个H₂O₂诱导基因中的22个。与Hik33相反，编码假定的过氧化物传感蛋白的PerR仅参与九个H₂O₂诱导基因的调控。
