Wang J F, Komarov P, Sies H, de Groot H
Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Federal Republic of Germany.
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):311-4. doi: 10.1042/bj2790311.
Phorbol 12-myristate 13-acetate-induced luminol chemiluminescence in rat Kupffer cells was doubled by the addition of L-arginine and significantly (up to 70%) inhibited by NG-nitro-L-arginine and NG-monomethyl-L-arginine, competitive inhibitors of L-arginine-dependent nitric oxide (NO) formation. The release of superoxide anion (O2-) by NADPH oxidase was neither affected by L-arginine nor by the inhibitors. Only very slight luminol chemiluminescence was detectable in lipopolysaccharide-pretreated Kupffer cells, a condition in which significant amounts of NO were formed but no O2-. In a cell-free system, significant luminol chemiluminescence only occurred when both authentic NO and the O2-/H2O2- generating system xanthine/xanthine oxidase were present. The results indicate that luminol chemiluminescence in phorbol-ester-activated Kupffer cells largely depends on L-arginine metabolism by NO synthase, requiring the concurrent formation of NO and O2-/H2O2.
佛波醇12 -肉豆蔻酸酯13 -乙酸酯诱导的大鼠库普弗细胞鲁米诺化学发光，在添加L -精氨酸后增加了一倍，而被L -精氨酸依赖性一氧化氮（NO）生成的竞争性抑制剂NG -硝基-L -精氨酸和NG -单甲基-L -精氨酸显著抑制（高达70%）。NADPH氧化酶释放超氧阴离子（O2-）既不受L -精氨酸影响，也不受抑制剂影响。在脂多糖预处理的库普弗细胞中只能检测到非常微弱的鲁米诺化学发光，在这种情况下会形成大量的NO但没有O2-。在无细胞系统中，只有当同时存在纯NO和产生O2-/H2O2的系统黄嘌呤/黄嘌呤氧化酶时，才会出现显著的鲁米诺化学发光。结果表明，佛波酯激活的库普弗细胞中的鲁米诺化学发光很大程度上依赖于一氧化氮合酶对L -精氨酸的代谢，需要同时形成NO和O2-/H2O2。
