Pellegrini Nicoletta, Salvatore Sara, Valtueña Silvia, Bedogni Giorgio, Porrini Marisa, Pala Valeria, Del Rio Daniele, Sieri Sabina, Miglio Cristiana, Krogh Vittorio, Zavaroni Ivana, Brighenti Furio
Department of Public Health, University of Parma, Parma, Italy.
J Nutr. 2007 Jan;137(1):93-8. doi: 10.1093/jn/137.1.93.
The total antioxidant capacity (TAC) of the diet may be an important tool to monitor the protective effect of plant foods in epidemiological studies. We developed a semi-quantitative FFQ for the assessment of dietary TAC by 3 different assays, i.e., Trolox equivalent antioxidant capacity (TEAC), total radical-trapping antioxidant parameter (TRAP) and ferric reducing-antioxidant power (FRAP). The FFQ consists of 53 questions about the major sources of dietary TAC in Northern Italy and was validated against a 3-d weighed food record (3D-WR) in 285 individuals (159 males and 126 females) aged 35-88 y and living in the province of Parma (Italy). Plasma TAC was also evaluated in a subgroup of subjects using the TEAC and FRAP assays. The FFQ was associated with 3D-WR (quadratic-weighted kappa = 0.49 for TEAC, 0.53 for TRAP, and 0.49 for FRAP; P < 0.0001) and proved reasonably accurate to classify individuals into quartiles of TAC intake. The FFQ had a good repeatability when readministered after 1 y in 55 subjects (quadratic-weighted kappa for intertertile agreement = 0.66 for TEAC, 0.70 for TRAP and 0.68 for FRAP; P < 0.0001). With both dietary instruments, the main contributors to TAC intake were coffee and tea in women and alcoholic beverages in men, followed by fruits and vegetables in both sexes. Plasma TAC and dietary TAC were not associated. In conclusion, our FFQ has the potential for being used to rank subjects on the basis of their antioxidant intake as determined by dietary TAC in large epidemiological studies. The FFQ should be validated in external populations before being used for research purposes.
在流行病学研究中，饮食的总抗氧化能力（TAC）可能是监测植物性食物保护作用的重要工具。我们开发了一种半定量食物频率问卷（FFQ），通过3种不同的分析方法，即Trolox等效抗氧化能力（TEAC）、总自由基捕获抗氧化参数（TRAP）和铁还原抗氧化能力（FRAP）来评估饮食TAC。该FFQ包含53个关于意大利北部饮食TAC主要来源的问题，并在285名年龄在35 - 88岁、居住在帕尔马省（意大利）的个体（159名男性和126名女性）中，以3天称重食物记录（3D - WR）为对照进行了验证。还使用TEAC和FRAP分析方法对一部分受试者的血浆TAC进行了评估。该FFQ与3D - WR相关（TEAC的二次加权kappa值为0.49，TRAP为0.53，FRAP为0.49；P < 0.0001），并且在将个体分类为TAC摄入量四分位数方面证明具有合理的准确性。在55名受试者1年后重新进行该FFQ测试时，其具有良好的重复性（TEAC的组间一致性二次加权kappa值为0.66，TRAP为0.70，FRAP为0.68；P < 0.0001）。使用这两种饮食工具时，女性TAC摄入量的主要贡献者是咖啡和茶，男性是酒精饮料，其次是男女两性的水果和蔬菜。血浆TAC与饮食TAC不相关。总之，我们的FFQ有潜力用于在大型流行病学研究中，根据饮食TAC确定的抗氧化剂摄入量对受试者进行排名。在用于研究目的之前，该FFQ应在外部人群中进行验证。
