开放通道噪声。VI. 用于确定快速动力学参数的幅度直方图分析
Open channel noise. VI. Analysis of amplitude histograms to determine rapid kinetic parameters.
作者信息
Heinemann S H, Sigworth F J
机构信息
Max-Planck-Institut für biophysikalische Chemie, Abteilung Membranbiophysik, Göttingen, Germany.
出版信息
Biophys J. 1991 Sep;60(3):577-87. doi: 10.1016/S0006-3495(91)82087-2.
Abstract
Recently we reported that rapid fluctuations of ion currents flowing through open gramicidin A channels exceed the expected level of pure transport noise at low ion concentrations (Heinemann, S. H. and F. J. Sigworth. 1990. Biophys. J. 57:499-514). Based on comparisons with kinetic ion transport models we concluded that this excess noise is likely caused by current interruptions lasting approximately 1 microsecond. Here we introduce a method using the higher-order cumulants of the amplitude distribution to estimate the kinetics of channel closing events far below the actual time resolution of the recording system. Using this method on data recorded with 10 kHz bandwidth, estimates for gap time constants on the order of 1 microsecond were obtained, similar to the earlier predictions.
摘要
最近我们报道，在低离子浓度下，流经开放短杆菌肽A通道的离子电流的快速波动超过了纯传输噪声的预期水平（海涅曼，S.H.和F.J.西格沃思。1990年。《生物物理学杂志》57:499 - 514）。基于与动力学离子传输模型的比较，我们得出结论，这种过量噪声可能是由持续约1微秒的电流中断引起的。在这里，我们介绍一种使用幅度分布的高阶累积量来估计远低于记录系统实际时间分辨率的通道关闭事件动力学的方法。对以10 kHz带宽记录的数据使用这种方法，获得了约1微秒量级的间隙时间常数估计值，与早期预测相似。
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本文引用的文献
1
Multioccupancy models for single filing ionic channels: theoretical behavior of a four-site channel with three barriers separating the sites.
J Membr Biol. 1983;71(1-2):61-78. doi: 10.1007/BF01870675.
2
Gramicidin channels.
Annu Rev Physiol. 1984;46:531-48. doi: 10.1146/annurev.ph.46.030184.002531.
3
The gramicidin A channel: a review of its permeability characteristics with special reference to the single-file aspect of transport.
J Membr Biol. 1981 Apr 30;59(3):155-71. doi: 10.1007/BF01875422.
4
Ion movements in gramicidin pores. An example of single-file transport.
Biochim Biophys Acta. 1980 Nov 4;602(2):331-54. doi: 10.1016/0005-2736(80)90316-8.
5
Ionic permeation and blockade in Ca2+-activated K+ channels of bovine chromaffin cells.
J Gen Physiol. 1984 Aug;84(2):157-86. doi: 10.1085/jgp.84.2.157.
6
Interactions of protons with single open L-type calcium channels. pH dependence of proton-induced current fluctuations with Cs+, K+, and Na+ as permeant ions.
J Gen Physiol. 1989 Jul;94(1):1-21. doi: 10.1085/jgp.94.1.1.
7
Estimation of Na+ dwell time in the gramicidin A channel. Na+ ions as blockers of H+ currents.
Biochim Biophys Acta. 1989 Dec 11;987(1):8-14. doi: 10.1016/0005-2736(89)90448-3.
8
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9
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J Physiol. 1988 Jan;395:131-59. doi: 10.1113/jphysiol.1988.sp016912.
10
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