离子通过短杆菌肽A通道的运动。界面极化对单通道电流测量的影响。
Ion movement through gramicidin A channels. Interfacial polarization effects on single-channel current measurements.
作者信息
Andersen O S
出版信息
Biophys J. 1983 Feb;41(2):135-46. doi: 10.1016/S0006-3495(83)84415-4.
Abstract
Gramicidin A single-channel current-voltage characteristics were studied at low permeant ion concentrations and very high applied potentials. The purpose of these experiments was to elucidate the basis for the small, but definite, voltage dependence observed under these circumstances. It was found that this residual voltage dependence is a reflection of interfacial polarization effects, similar to those proposed by Walz et al. (Biophys. J. 9:1150-1159). It will be concluded that there exists an effectively voltage-independent step in the association reaction between a gramicidin A channel and the permeating ion. Some consequences of interfacial polarization effects for the analysis of conductance vs. activity relations will be discussed.
摘要
短杆菌肽A单通道电流-电压特性在低渗透离子浓度和非常高的外加电位下进行了研究。这些实验的目的是阐明在这些情况下观察到的虽小但确定的电压依赖性的基础。结果发现，这种残余电压依赖性是界面极化效应的反映，类似于瓦尔兹等人提出的效应（《生物物理杂志》9:1150 - 1159）。可以得出结论，在短杆菌肽A通道与渗透离子之间的缔合反应中存在一个有效的电压非依赖性步骤。将讨论界面极化效应对于电导与活性关系分析的一些后果。
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