Samperi P, Mancuso G R, Dibenedetto S P, Di Cataldo A, Ragusa R, Schilirò G
Cattedra di Ematologia Pediatrica, Università di Catania, Italy.
Clin Lab Haematol. 1991;13(2):169-75. doi: 10.1111/j.1365-2257.1991.tb00266.x.
Differentiation of some abnormal haemoglobins, such as Hb C, O-Arab, Agenogi, E, O-Indonesia, C-Harlem, and Siriraj, is difficult and quantitation of the various fractions is impossible with cellulose acetate electrophoresis. The authors report 13 cases of Hb C, 10 of Hb O-Arab and 5 of Hb Agenogi whose haemoglobin fractions were quantitated by HPLC during a thalassaemia screening programme. Hb F was determined by both Betke's method and HPLC. Analysis of data by linear regression demonstrates that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows quantitative and qualitative discrimination of the various haemoglobin fractions, making it a valid tool in screening programmes for haemoglobinopathies.
鉴别某些异常血红蛋白，如Hb C、O-阿拉伯、阿戈诺吉、E、O-印度尼西亚、C-哈勒姆和诗里拉杰型血红蛋白是困难的，用醋酸纤维素电泳无法对各种组分进行定量分析。作者报告了13例Hb C、10例Hb O-阿拉伯和5例Hb阿戈诺吉患者，他们的血红蛋白组分在一项地中海贫血筛查计划中通过高效液相色谱法（HPLC）进行了定量分析。Hb F通过贝特克法和HPLC法测定。通过线性回归对数据进行分析表明，这两种方法得出的结果相互重叠。我们的研究结果表明，HPLC是一种快速且易于重复的方法，能够对各种血红蛋白组分进行定量和定性鉴别，使其成为血红蛋白病筛查计划中的一种有效工具。
