Thomas S
Department of Pathology, Christian Medical College Hospital, Vellore, India.
J Clin Pathol. 1998 Mar;51(3):253-4. doi: 10.1136/jcp.51.3.253.
High performance liquid chromatography (HPLC) is increasingly being used to estimate variant haemoglobins. A case of haemoglobin S/D (HbS/D) is presented, which was misdiagnosed as haemoglobin S/E (HbS/E) by HPLC. The patient was a 22 year old woman with sickle cell anaemia. Subsequent haemodilution by blood transfusion clearly elucidated the haemoglobin D peak on HPLC. Sample dilution experiments, using the pretransfusion sample, were done resulting in correct elution of the peak in the D window. Troubleshooting in similar problematic haemoglobin variant peaks seen on HPLC can be done by sample dilution.
高效液相色谱法（HPLC）越来越多地用于估算变异血红蛋白。本文报告了一例血红蛋白S/D（HbS/D）病例，该病例被HPLC误诊为血红蛋白S/E（HbS/E）。患者为一名患有镰状细胞贫血的22岁女性。随后通过输血进行的血液稀释清楚地显示了HPLC上的血红蛋白D峰。使用输血前样本进行了样本稀释实验，结果在D窗口中峰的洗脱正确。对于HPLC上出现的类似有问题的血红蛋白变异峰，可以通过样本稀释进行故障排除。
