Baumann H, Schendel P
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263.
J Biol Chem. 1991 Oct 25;266(30):20424-7.
Treatment of rat hepatoma H-35 cells with purified human recombinant interleukin-11 (IL-11) resulted in the stimulated production of several major acute phase plasma proteins. The qualitative and quantitative changes were comparable to those mediated by IL-6 or leukemia inhibitor factor (LIF). Like IL-6, IL-11 acted synergistically with IL-1 on type 1 acute phase proteins. The combination of IL-11 and dexamethasone yielded a magnitude of stimulation which was more similar to the combination of LIF and dexamethasone than IL-6 and dexamethasone. IL-11 elicited in treatment of primary cultures of rat hepatocytes a qualitative change of plasma protein production which was similar to that in H-35 cells. Comparison of rat and human hepatoma cells indicated that the IL-11 response did not correlate with that of IL-6 or LIF, suggesting that the action of IL-11 was mediated by an IL-11-specific receptor system. However, the intracellular transduction of the IL-11, IL-6, and LIF signals to the acute phase protein genes seems to rely, in part, on common elements as judged from their stimulatory effects on the transfected expression vector containing the IL-6 response element of the rat beta-fibrinogen gene. The finding that IL-11 shares liver-regulating properties with IL-6 and LIF suggests that IL-11 has the potential of contributing to the control of systemic homeostasis and hepatic acute phase response.
用纯化的人重组白细胞介素-11（IL-11）处理大鼠肝癌H-35细胞，可刺激几种主要急性期血浆蛋白的产生。其定性和定量变化与IL-6或白血病抑制因子（LIF）介导的变化相当。与IL-6一样，IL-11与IL-1对1型急性期蛋白起协同作用。IL-11与地塞米松联合产生的刺激程度，与LIF和地塞米松联合相比，比IL-6和地塞米松联合更相似。IL-11在处理大鼠肝细胞原代培养物时，引起血浆蛋白产生的定性变化，这与H-35细胞中的情况相似。大鼠和人肝癌细胞的比较表明，IL-11反应与IL-6或LIF的反应不相关，这表明IL-11的作用是由IL-11特异性受体系统介导的。然而，从它们对含有大鼠β-纤维蛋白原基因IL-6反应元件的转染表达载体的刺激作用判断，IL-11、IL-6和LIF信号向急性期蛋白基因的细胞内转导似乎部分依赖于共同元件。IL-11与IL-6和LIF具有共同的肝脏调节特性这一发现表明，IL-11有潜力参与全身内环境稳态和肝脏急性期反应的调控。
