Tansey T, Schultz J R, Miller R C, Storti R V
Department of Biochemistry, University of Illinois College of Medicine, Chicago 60612.
Mol Cell Biol. 1991 Dec;11(12):6337-42. doi: 10.1128/mcb.11.12.6337-6342.1991.
The effects of promoter deletions on Drosophila tropomyosin I (TmI) gene expression have been determined by measuring TmI RNA levels in transformed flies. Decreases in RNA levels have been correlated with rescue of flightless and jumpless mutant phenotypes in Ifm(3)3 mutant transformed flies and changes in muscle ultrastructure. The results of this analysis have allowed us to identify a region responsible for 20% of maximal TmI expression, estimate threshold levels of TmI RNA required for indirect flight and jump muscle function, and obtain evidence suggesting that sarcomere length may be an important determinant of flight muscle function.
通过测量转基因果蝇中肌动蛋白抑制蛋白I（TmI）的RNA水平，已确定启动子缺失对果蝇肌动蛋白抑制蛋白I（TmI）基因表达的影响。RNA水平的降低与Ifm(3)3突变转基因果蝇中无飞行能力和无跳跃能力突变表型的挽救以及肌肉超微结构的变化相关。该分析结果使我们能够确定一个负责最大TmI表达20%的区域，估计间接飞行和跳跃肌肉功能所需的TmI RNA阈值水平，并获得证据表明肌节长度可能是飞行肌肉功能的重要决定因素。
