Spradling A C, Rubin G M
Science. 1982 Oct 22;218(4570):341-7. doi: 10.1126/science.6289435.
Recombinant DNA carrying the 3-kilobase transposable element was injected into Drosophila embryos of a strain that lacked such elements. Under optimum conditions, half of the surviving embryos showed evidence of P element-induced mutations in a fraction of their progeny. Direct analysis of the DNA of strains derived from such flies showed them to contain from one to five intact 3-kilobase P elements located at a wide variety of chromosomal sites. DNA sequences located outside the P element on the injected DNA were not transferred. Thus P elements can efficiently and selectively transpose from extrachromosomal DNA to the DNA of germ line chromosomes in Drosophila embryos. These observations provide the basis for efficient DNA-mediated gene transfer in Drosophila.
携带3千碱基转座元件的重组DNA被注射到一个缺乏此类元件的果蝇品系的胚胎中。在最佳条件下，一半存活的胚胎在其部分后代中显示出P元件诱导突变的证据。对源自此类果蝇的品系的DNA进行直接分析表明，它们含有1至5个完整的3千碱基P元件，位于各种各样的染色体位点。注射的DNA上位于P元件之外的DNA序列未被转移。因此，P元件可以在果蝇胚胎中有效地、选择性地从染色体外DNA转座到种系染色体的DNA中。这些观察结果为果蝇中高效的DNA介导的基因转移提供了基础。
