Freitas Luciana S, Freitas Tiago P, Silveira Paulo C, Rocha Luís G, Pinho Ricardo A, Streck Emilio L
Laboratório de Fisiopatologia Experimental, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil.
Cell Biol Int. 2007 May;31(5):482-8. doi: 10.1016/j.cellbi.2006.11.015. Epub 2006 Nov 28.
Contusion injuries are a very common form of both athletic and non-athletic injury, that effect muscle function. Treatments to augment the normal repair and regeneration processes are important for a wide variety of patients. Therapeutic ultrasound has been claimed to promote tissue repair, especially by enhancing cell proliferation and protein synthesis. The present study aimed to investigate the effect of therapeutic pulsed ultrasound (TPU) on parameters of oxidative stress, namely thiobarbituric acid-reactive substances (TBARS), protein carbonyl content and the activities of antioxidant enzymes, catalase and superoxide dismutase (SOD), in skeletal muscle after injury. Wistar rats were submitted to an animal model of muscle (gastrocnemius) laceration. TPU was used once a day. One, three or five days after muscle laceration, the animals were killed by decapitation and oxidative stress parameters were evaluated. Serum CK levels were increased in muscle-injured animals, indicating that the laceration animal model was successful. TBARS were not altered after muscle injury, when compared to the sham group. Protein carbonyl content was increased after muscle laceration. Catalase and SOD activities were increased 1 day after muscle injury and not altered at days 3 and 5. TPU decreased TBARS levels after muscle laceration when compared to injured muscle animals without treatment. Protein carbonyl content evaluation presented similar results. It is tempting to speculate that TPU seems to protect the tissue from oxidative injury. TPU diminished catalase and SOD activities, especially on the first day following muscle laceration.
挫伤是运动损伤和非运动损伤中非常常见的一种形式，会影响肌肉功能。对于众多患者而言，增强正常修复和再生过程的治疗方法至关重要。治疗性超声据称可促进组织修复，特别是通过增强细胞增殖和蛋白质合成来实现。本研究旨在探讨治疗性脉冲超声（TPU）对损伤后骨骼肌氧化应激参数的影响，这些参数包括硫代巴比妥酸反应性物质（TBARS）、蛋白质羰基含量以及抗氧化酶过氧化氢酶和超氧化物歧化酶（SOD）的活性。将Wistar大鼠用于建立肌肉（腓肠肌）撕裂伤的动物模型。每天使用一次TPU。在肌肉撕裂伤后的第1、3或5天，通过断头处死动物并评估氧化应激参数。肌肉损伤的动物血清CK水平升高，表明撕裂伤动物模型构建成功。与假手术组相比，肌肉损伤后TBARS未发生改变。肌肉撕裂伤后蛋白质羰基含量增加。肌肉损伤后第1天过氧化氢酶和SOD活性增加，而在第3天和第5天未发生改变。与未接受治疗的肌肉损伤动物相比，TPU降低了肌肉撕裂伤后的TBARS水平。蛋白质羰基含量评估结果相似。由此推测，TPU似乎能保护组织免受氧化损伤。TPU降低了过氧化氢酶和SOD的活性，尤其是在肌肉撕裂伤后的第一天。
