Laboratório de Bioenergética e Estresse Oxidativo/UFSC, Campus Universitário, Bloco C, 2° andar, Salas 201/214, CEP: 88040-900 Florianópolis, SC, Brazil.
Ultrasonics. 2012 Jul;52(5):650-4. doi: 10.1016/j.ultras.2012.01.007. Epub 2012 Jan 26.
The aim of the study was to evaluate the effects of TPU together with DMSO on oxidative stress parameters after eccentric exercise.
Thirty and six animals were divided in control; eccentric exercise (EE); EE+saline gel 0.9%; EE+TPU 0.8 W/cm(2); EE+DMSO gel; EE+TPU+DMSO gel and submitted to one 90-min downhill run (1.0 km h(-1)). TPU was used 2, 12, 24, 46 h after exercise session and 48 h after the animals were killed and the gastrocnemius muscles were surgically removed. Production of superoxide anion, creatine kinase (CK) levels, lipoperoxidation, carbonylation, and antioxidants enzymes were analyzed.
Showed that TPU and gel-DMSO improved muscle healing. Moreover, superoxide anion production, TBARS level and protein carbonyls levels, superoxide dismutase and catalase activity were all decreased in the group TPU plus gel-DMSO.
Our results show that DMSO is effective in the reduction of the muscular lesion and in the oxidative stress after eccentric exercise only when used with TPU.
本研究旨在评估 TPU 联合 DMSO 对离心运动后氧化应激参数的影响。
将 30 只和 6 只动物分为对照组、离心运动（EE）组、EE+生理盐水凝胶 0.9%组、EE+TPU 0.8 W/cm(2)组、EE+DMSO 凝胶组、EE+TPU+DMSO 凝胶组，并进行 1 次 90 分钟下坡跑（1.0 km h(-1)）。TPU 在运动后 2、12、24、46 小时使用，动物处死和腓肠肌切除后 48 小时进行。分析超氧阴离子生成、肌酸激酶（CK）水平、脂质过氧化、羰基化和抗氧化酶。
表明 TPU 和凝胶-DMSO 改善了肌肉愈合。此外，TPU 加凝胶-DMSO 组超氧阴离子生成、TBARS 水平和蛋白质羰基水平、超氧化物歧化酶和过氧化氢酶活性均降低。
我们的结果表明，DMSO 只有与 TPU 联合使用时，才能有效减少离心运动后的肌肉损伤和氧化应激。
