Shingel Kirill I, Di Stabile Liliana, Marty Jean-Paul, Faure Marie-Pierre
Bioartificial Gel Technologies Inc., Montreal, Quebec, Canada H3A 1L4.
Int Wound J. 2006 Dec;3(4):332-42. doi: 10.1111/j.1742-481X.2006.00262.x.
In this study, a novel soft hydrogel system based on the poly(ethylene glycol)-protein conjugates was evaluated as an occlusive wound dressing material. The hydrogel material, referred by the name of BioAquacare, contains up to 96% of the liquid and is formulated with phosphate-buffered saline and safe preservative to control bacterial load in the open wounds. Performance of the BioAquacare as a wound dressing material was assessed in partial- and full-thickness wounds in pigs. Wound analysis comprised macroscopic determination of the wound size, histological examination of the healing tissues and biochemical characterisation of wound exudates. The wounds treated with BioAquacare healed without any signs of inflammation, skin irritation, oedema or erythema. Cellular composition of the reepithelialised wounds was very similar to that of the normal skin, with a well-developed stratum corneum and epithelial layer. It was observed that BioAquacare plays the role of a liquid compartment, which provides pronounced hydration effect and helps maintain a natural moist environment of the healing tissues. BioAquacare showed relatively low protein-absorbing activity, absorbing predominantly low-molecular-weight molecules, including interleukin (IL)-1beta, IL-6, transforming growth factor-beta1 and products of haemoglobin degradation. It is concluded that application of the moist BioAquacare dressing promotes fast reepithelialisation by creating favourable environment for keratinocytes proliferation and it also reduces scarring. The results show that BioAquacare can be considered as a safe, biocompatible and inflammatory inert wound dressing material.
在本研究中，一种基于聚乙二醇 - 蛋白质缀合物的新型软质水凝胶系统被评估为一种封闭性伤口敷料材料。这种名为BioAquacare的水凝胶材料含有高达96%的液体，由磷酸盐缓冲盐水和安全防腐剂配制而成，以控制开放性伤口中的细菌载量。在猪的部分厚度和全厚度伤口中评估了BioAquacare作为伤口敷料材料的性能。伤口分析包括伤口大小的宏观测定、愈合组织的组织学检查以及伤口渗出物的生化表征。用BioAquacare治疗的伤口愈合时没有任何炎症、皮肤刺激、水肿或红斑的迹象。重新上皮化伤口的细胞组成与正常皮肤非常相似，具有发育良好的角质层和上皮层。据观察，BioAquacare起到了液体隔室的作用，提供显著的水合作用，并有助于维持愈合组织的自然湿润环境。BioAquacare显示出相对较低的蛋白质吸收活性，主要吸收低分子量分子，包括白细胞介素（IL）-1β、IL-6、转化生长因子-β1和血红蛋白降解产物。得出的结论是，使用湿润的BioAquacare敷料通过为角质形成细胞增殖创造有利环境来促进快速重新上皮化，并且还减少疤痕形成。结果表明，BioAquacare可被视为一种安全、生物相容且无炎症反应的伤口敷料材料。
