Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
National Institute of Laser Enhanced Sciences, Cairo University, Giza, 12613, Egypt.
Lasers Med Sci. 2024 May 27;39(1):141. doi: 10.1007/s10103-024-04084-1.
Conventional approaches for enhancing wound healing may not always yield satisfactory results. Instead, we test the effectiveness of a newly developed photodynamic therapy (PDT) that uses methylene blue (MB) loaded with polyethylene glycol (PEG) (MB-PEG) hydrogel to accelerate wound healing process in mice.
A dorsal skin incision with 6 mm punch which topically subjected to MB-PEG hydrogel and a low-level laser light of red light to assess the regeneration process of wounded skin. A total of 63 adult male CD1 mice divided into normal group (no treatment) and other wound groups received different treatments of laser (650 ± 5 nm and power intensity of 180 mW/cm), MB-PEG, or PDT (MB-PEG followed by laser). The wound healing parameters were investigated by histological examination of the skin and measuring of proinflammatory cytokines at the early stage (48 h) and a late one on day 21.
at 48 h, the score of tissue granulation, inflammation, and angiogenesis process were markedly improved in wounded groups that received MB + PEG combined with laser compared to the group treated with laser alone. On day 21, a significant improvement of the inflammation was detected in the group treated with MB + PEG plus laser compared to the other groups. At 48 h, the upregulated serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in the wound group were significantly (P < 0.001) reduced in the group treated with MB + PEG combined with laser.
MB-PEG based hydrogel improves and accelerates wound closure in the context of laser compared to either single treatment.
传统的促进伤口愈合的方法并不总是能产生令人满意的结果。因此，我们测试了一种新的光动力疗法（PDT）的有效性，该疗法使用载有聚乙二醇（PEG）的亚甲蓝（MB）水凝胶（MB-PEG）来加速小鼠的伤口愈合过程。
用 6mm 打孔器在背部皮肤做一个切口，局部应用 MB-PEG 水凝胶和低水平的红光激光，以评估受伤皮肤的再生过程。将 63 只成年雄性 CD1 小鼠分为正常组（无治疗）和其他伤口组，分别接受激光（650±5nm，功率强度为 180mW/cm）、MB-PEG 或 PDT（MB-PEG 后激光）的不同治疗。通过皮肤组织学检查和早期（48h）和晚期（第 21 天）测量促炎细胞因子，研究伤口愈合参数。
在 48h 时，与单独接受激光治疗的组相比，接受 MB+PEG 联合激光治疗的伤口组的组织肉芽形成、炎症和血管生成过程评分明显提高。在第 21 天，与其他组相比，接受 MB+PEG 加激光治疗的组的炎症明显改善。在 48h 时，伤口组血清肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β水平升高，接受 MB+PEG 联合激光治疗的组显著降低（P<0.001）。
与单一治疗相比，MB-PEG 水凝胶在激光照射下能改善和加速伤口闭合。
