Sánchez L, Martínez P, Goyanes V
Departamento de Biología Fundamental, Universidad de Santiago, Lugo, Spain.
Genome. 1991 Oct;34(5):710-3. doi: 10.1139/g91-109.
Human chromosomes were treated with 5-azacytidine and analyzed by whole-mount electron microscopy. This base analogue produces undercondensation of heterochromatin and separation of the centromere from the bulk of pericentromeric heterochromatin in chromosomes 1, 9, 15, and 16, which allows clear delimitation of the centromere regions. A quantitative analysis of centromeres showed that chromosomes 1, 9, and 16 have centromeres of different size. The centromere of chromosome 15 is similar in size to that of chromosome 9 and different from those of chromosomes 1 and 16. No interindividual variation for centromere size was found. A positive correlation between centromere and chromosome size was found for the chromosomes analyzed.
人类染色体用5-氮杂胞苷处理后，通过整装电子显微镜进行分析。这种碱基类似物会导致异染色质凝聚不足，以及1号、9号、15号和16号染色体的着丝粒与大部分着丝粒周围异染色质分离，这使得着丝粒区域能够清晰界定。对着丝粒的定量分析表明，1号、9号和16号染色体有着不同大小的着丝粒。15号染色体的着丝粒大小与9号染色体相似，与1号和16号染色体不同。未发现着丝粒大小存在个体间差异。在所分析的染色体中，发现着丝粒大小与染色体大小之间存在正相关。
