Fernández J L, Goyanes V, Pereira S, López-Fernández C, Gosálvez J
Departamento de Biología, Universidad Autónoma de Madrid, Spain.
Chromosome Res. 1994 Jan;2(1):29-35. doi: 10.1007/BF01539451.
Fluorescence in situ hybridization employing human alphoid, beta and classical satellite DNA probes was performed on 5-azacytidine treated and untreated chromosomes obtained from human lymphocytes. The individual used in this study presented a polymorphism of constitutive heterochromatin of chromosomes 1 and 9 as revealed by in situ digestion with the restriction endonuclease Alul. Neither the alphoid nor the beta satellite DNA domains were susceptible to condensation-inhibition by 5-azacytidine. Only the classical satellite localized on chromosome 9 was affected. The constitutive heterochromatin size polymorphism was shown to depend mainly on variations of the classical satellite DNA domain. Therefore, condensation-inhibition, as a phenomenon which may modify the natural folding of the chromatin fibre, regionally affects human constitutive heterochromatin and seems to be dependent on the heterochromatic family.
采用人α卫星、β卫星和经典卫星DNA探针，对来自人淋巴细胞的经5-氮杂胞苷处理和未处理的染色体进行荧光原位杂交。本研究中使用的个体经限制性内切酶Alul原位消化显示，1号和9号染色体的组成型异染色质存在多态性。α卫星和β卫星DNA结构域均不易受到5-氮杂胞苷的凝聚抑制作用。仅位于9号染色体上的经典卫星受到影响。组成型异染色质大小多态性主要取决于经典卫星DNA结构域的变化。因此，凝聚抑制作为一种可能改变染色质纤维自然折叠的现象，局部影响人类组成型异染色质，且似乎取决于异染色质家族。
