Tabibzadeh S
Department of Pathology, City Hospital Center, Elmhurst, New York 11373.
J Clin Endocrinol Metab. 1991 Dec;73(6):1352-9. doi: 10.1210/jcem-73-6-1352.
Endometrial epithelial cells express HLA-DR molecules of the major histocompatibility complex in vivo adjacent to aggregates of T-cells in the endometrial stroma. To test whether HLA-DR expression on endometrial epithelium is mediated by T-cells, endometrial T-cells were isolated from human endometrium by the sheep red blood cell rosetting technique. In contrast to the resting T-cells from peripheral blood and similar to the peripheral blood T-cells activated with Concanavalin-A, endometrial T-cells formed colonies in vitro. Direct addition of the endometrial T-cells to epithelial cell cultures derived from autologous glands induced both morphological changes as well as HLA-DR molecules in the epithelial cells. The intensity of the immunostained HLA-DR molecules in the epithelial cells as well as the percentages of the HLA-DR-positive epithelial cells correlated with the number of T-cells added to the epithelial cell cultures. T-Cells were bound to the HLA-DR-positive epithelial cells as single cells or aggregates, and they were not found bound to the HLA-DR-negative epithelial cells. The supernatant of the endometrial T-cells induced expression of HLA-DR molecules and morphological changes in cultures of HLA-DR-negative epithelial cells. This expression could be inhibited by a neutralizing antiserum to interferon-gamma. These findings are consistent with the hypothesis that endometrial T-cells are activated and suggest that the expression of HLA-DR molecules in glandular epithelium in vivo is mediated by the interferon-gamma secreted by the endometrial T-cells.
在体内，子宫内膜上皮细胞在子宫内膜基质中与T细胞聚集物相邻处表达主要组织相容性复合体的HLA - DR分子。为了检测子宫内膜上皮细胞上HLA - DR的表达是否由T细胞介导，采用绵羊红细胞花环技术从人子宫内膜中分离出子宫内膜T细胞。与外周血中的静止T细胞不同，类似于用伴刀豆球蛋白A激活的外周血T细胞，子宫内膜T细胞在体外形成集落。将子宫内膜T细胞直接添加到源自自体腺体的上皮细胞培养物中，可诱导上皮细胞发生形态变化以及表达HLA - DR分子。上皮细胞中免疫染色的HLA - DR分子强度以及HLA - DR阳性上皮细胞的百分比与添加到上皮细胞培养物中的T细胞数量相关。T细胞以单个细胞或聚集物的形式与HLA - DR阳性上皮细胞结合，未发现它们与HLA - DR阴性上皮细胞结合。子宫内膜T细胞的上清液可诱导HLA - DR阴性上皮细胞培养物中HLA - DR分子的表达和形态变化。这种表达可被抗干扰素 - γ的中和抗血清抑制。这些发现与子宫内膜T细胞被激活的假说一致，并表明体内腺上皮中HLA - DR分子的表达是由子宫内膜T细胞分泌的干扰素 - γ介导的。
