Polak Jan, Kovacova Zuzana, Jacek Martin, Klimcakova Eva, Kovacikova Michaela, Vitkova Michaela, Kuda Ondrej, Sebela Marek, Samcova Eva, Stich Vladimir
Department of Sport Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Clin Sci (Lond). 2007 Jun;112(11):557-65. doi: 10.1042/CS20060296.
Adiponectin is involved in the regulation of glucose and fatty acid metabolism, influences whole-body insulin sensitivity and protects arterial walls against the development of atherosclerosis. Plasma adiponectin is decreased in obese, insulin-resistant and Type 2 diabetic patients. Adiponectin circulates in plasma as high-, medium- and low-molecular-weight ('mass') forms (HMW, MMW and LMW respectively). The HMW form is believed to be closely associated with insulin sensitivity. The aim of the present study was to investigate whether diet-induced changes in body weight and insulin sensitivity were associated with changes in the quantity of adiponectin multimeric complexes. A total of 20 overweight or obese women (age, 39.4+/-9.5 years; body mass index, 32.2+/-6.4 kg/m(2)) underwent 12 weeks of low caloric diet (600 kcal/day less than energy requirements; where 1 kcal is approximately 4.184 kJ). Plasma samples were drawn before and after the study for biochemical analysis and Western blot detection of adiponectin multimeric complexes. The hypocaloric diet resulted in a weight reduction (89.8+/-16.4 kg compared with 83.1+/-15.6 kg; P<0.001) and an improvement in whole-body insulin sensitivity, as measured by HOMA (homoeostasis model assessment index; 1.9+/-0.8 compared with 1.5+/-0.7; P=0.013). Increases in the quantities of the HMW, MMW and LMW forms by 5.5, 8.5 and 18.1% respectively, were observed (P<0.05 for all of the forms). Total plasma adiponectin was increased by 36% with borderline significance (P=0.08). No correlations between changes in adiponectin complexes and changes in indices of insulin sensitivity were observed. In conclusion, diet-induced weight loss improved insulin sensitivity as well as increased the amount of HMW, MMW and LMW adiponectin complexes in plasma.
脂联素参与葡萄糖和脂肪酸代谢的调节，影响全身胰岛素敏感性，并保护动脉壁免受动脉粥样硬化的发展。肥胖、胰岛素抵抗和2型糖尿病患者的血浆脂联素水平降低。脂联素以高、中、低分子量（“质量”）形式（分别为HMW、MMW和LMW）在血浆中循环。HMW形式被认为与胰岛素敏感性密切相关。本研究的目的是调查饮食引起的体重和胰岛素敏感性变化是否与脂联素多聚体复合物数量的变化有关。共有20名超重或肥胖女性（年龄39.4±9.5岁；体重指数32.2±6.4kg/m²）接受了12周的低热量饮食（比能量需求少600千卡/天；1千卡约为4.184千焦）。在研究前后采集血浆样本，用于生化分析和脂联素多聚体复合物的蛋白质印迹检测。低热量饮食导致体重减轻（从89.8±16.4千克降至83.1±15.6千克；P<0.001），并改善了全身胰岛素敏感性，通过HOMA（稳态模型评估指数）测量（从1.9±0.8降至1.5±0.7；P=0.013）。观察到HMW、MMW和LMW形式的数量分别增加了5.5%、8.5%和18.1%（所有形式P<0.05）。血浆总脂联素增加了36%，具有临界显著性（P=0.08）。未观察到脂联素复合物变化与胰岛素敏感性指标变化之间的相关性。总之，饮食引起的体重减轻改善了胰岛素敏感性，并增加了血浆中HMW、MMW和LMW脂联素复合物的量。
