Esbrit P, García-Ocaña A, García-Cañero R, Manzano F, Jiménez-Clavero M A
Laboratorio de la Unidad Metabólica, Fundación Jiménez Díaz-CSIC, Madrid, España.
Ren Physiol Biochem. 1991 Nov-Dec;14(6):224-35. doi: 10.1159/000173410.
The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4: 334-338, 1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.
对来自单侧肾切除大鼠血浆的一种肾脏生长因子（KGF）的生物学特性进行了研究。该因子的粗制品[《肾透析与移植》4: 334 - 338, 1989]通过疏水相互作用高效液相色谱法和凝胶过滤进一步纯化。发现KGF是一种耐热且耐胰蛋白酶的蛋白质。该因子在体内能剂量依赖性地刺激小鼠肾脏的DNA合成，在体外能刺激大鼠肾小管或血清饥饿的LLC - PK1细胞的DNA合成。KGF还能以剂量依赖性方式增加这些细胞中的蛋白质合成。此外，KGF刺激这些细胞摄取钠，这与蛋白质合成的最大增加相关。我们的研究结果表明，KGF是一种强效的促肾生长蛋白，在单侧肾切除后的肾脏代偿性生长中可能起关键作用。
