García-Ocaña A, Ortega J, González-García Y, García-Cantón C, Esbrit P
Metabolic Unit Research Laboratory, Fundación Jiménez-Díaz, Madrid, Spain.
Nephron. 1993;64(4):547-51. doi: 10.1159/000187398.
A renotropic factor was partially purified by sequential gel filtration and anion exchanger chromatography from plasma of human kidney transplantation donors and a renal cancer patient after uninephrectomy. This activity increased the rate of [3H]thymidine incorporation into DNA in rat cortical tubules, but not in rat liver cells, within the range of 100-200 ng/ml protein. The renotropic activity was detected between 7 and 12 days after uninephrectomy, and at least in 1 case decreased thereafter. This activity was undetected in gel-filtrated plasma of patients after a nonurological surgical procedure. The potency of this renotropic activity and its elution by gel filtration are similar to those displayed by a renal growth factor activity isolated from uninephrectomized rat plasma, as recently reported.
通过连续凝胶过滤和阴离子交换柱色谱法，从人肾移植供体以及肾切除术后的一名肾癌患者的血浆中部分纯化出一种促肾生长因子。在蛋白质浓度为100 - 200 ng/ml范围内，该活性可提高大鼠皮质肾小管中[3H]胸腺嘧啶核苷掺入DNA的速率，但对大鼠肝细胞无此作用。促肾生长活性在肾切除术后7至12天可检测到，至少有1例患者此后该活性降低。在接受非泌尿外科手术的患者经凝胶过滤的血浆中未检测到该活性。这种促肾生长活性的效力及其通过凝胶过滤的洗脱情况，与最近报道的从单侧肾切除大鼠血浆中分离出的肾生长因子活性相似。
