一种用于血管紧张素转换酶（激酶II）的灵敏放射化学测定法。
A sensitive radiochemical assay for angiotensin-converting enzyme (kinase II).
作者信息
Chiu A T, Ryan J W, Ryan U S
出版信息
Biochem J. 1975 Jul;149(1):297-300. doi: 10.1042/bj1490297.
Abstract
[[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells.
摘要
[125I]酪氨酸8]缓激肽被血管紧张素转换酶降解为[125I]酪氨酸-精氨酸。反应产物可通过阳离子交换色谱法与未反应的底物完全分离并几乎定量回收。因此，有可能使用高比放射性（约400居里/毫摩尔）的[125I]酪氨酸8]缓激肽来测量肺内皮细胞小规模培养物中遇到的少量血管紧张素转换酶。
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