用重氮对玫瑰苯胺改良冷冻淋巴造血组织切片的固定。
Improved fixation of frozen lympho-haemopoietic tissue sections with hexazotized pararosaniline.
作者信息
De Jong J P, Voerman J S, Leenen P J, Van der Sluijs-Gelling A J, Ploemacher R E
机构信息
Department of Cell Biology, Erasmus University, Rotterdam, The Netherlands.
出版信息
Histochem J. 1991 Sep;23(9):392-401. doi: 10.1007/BF01042295.
In this study, a simple single-step fixation method for frozen tissue sections is introduced using the hexazotized salt of Pararosaniline as preservative agent. Tissue preservation by this method was shown to be superior to the commonly-used fixation with acetone. Fixation with hexazotized Pararosaniline caused a minimal loss of antigenicity as demonstrated using twenty-three monoclonal antibodies directed against lympho-haemopoietic and stromal cells.
在本研究中，介绍了一种使用副蔷薇苯胺重氮盐作为防腐剂的冷冻组织切片单步简易固定方法。结果表明，该方法对组织的保存效果优于常用的丙酮固定法。使用针对淋巴造血细胞和基质细胞的23种单克隆抗体进行检测，结果显示用副蔷薇苯胺重氮盐固定导致的抗原性损失最小。
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