Hirsch S, Austyn J M, Gordon S
J Exp Med. 1981 Sep 1;154(3):713-25. doi: 10.1084/jem.154.3.713.
We have defined the expression of the macrophages (m phi)-specific antigen (Ag) F4/80 during differentiation in culture. The progenitor cells-the colony-forming unit in culture and cluster-forming cell-lacked Ag F4/80 but gave rise to colonies of F4/80-positive adherent m phi, as shown by fluorescence-activated cell sorting and clonal assays with L cell-conditioned medium as the source of growth factor. Ag F4/80 first appeared on a nonadherent precursor found in mass liquid BM cultures after 3 d. Once adherent, m phi expressed high levels of Ag F4/80 and other markers. The role of L cell-conditioned medium and of adherence on expression of Ag F4/80 was also examined. Clonal analysis of F4/80 and other Ag, Mac-1, and 2.4G2 (FcR) showed that all cells in all independent colonies come to express these markers. These studies establish that F4/80 is a marker for the more mature stages of m phi development and that Ag expression increases progressively during maturation in vitro. Heterogeneity of Ag expression can be ascribed to variation in development and not to independent subsets of the m phi.
我们已经确定了巨噬细胞（m phi）特异性抗原（Ag）F4/80在体外培养分化过程中的表达情况。祖细胞——培养中的集落形成单位和簇形成细胞——缺乏Ag F4/80，但能产生F4/80阳性贴壁巨噬细胞集落，荧光激活细胞分选以及以L细胞条件培养基作为生长因子来源的克隆分析均证明了这一点。Ag F4/80首先出现在第3天后大量液体骨髓培养物中发现的非贴壁前体细胞上。一旦贴壁，巨噬细胞就会高表达Ag F4/80和其他标志物。我们还研究了L细胞条件培养基和贴壁对Ag F4/80表达的作用。对F4/80和其他抗原、Mac-1以及2.4G2（FcR）的克隆分析表明，所有独立集落中的所有细胞都会表达这些标志物。这些研究表明，F4/80是巨噬细胞发育更成熟阶段的标志物，并且在体外成熟过程中抗原表达会逐渐增加。抗原表达的异质性可归因于发育差异，而非巨噬细胞的独立亚群。
