Alavi Seyed Mehdi, Poussier Stéphane, Manceau Charles
UMR PaVé, centre INRA, 42 rue Georges Morel, 49071 Beaucouzé Cedex 1, France.
Appl Environ Microbiol. 2007 Mar;73(5):1678-82. doi: 10.1128/AEM.02031-06. Epub 2007 Jan 5.
ISXax1 is a novel insertion sequence belonging to the IS256 and Mutator families. Dot blot, Southern blot, and PCR analyses revealed that ISXax1 is restricted to Xanthomonas axonopodis pv. phaseoli (variants fuscans and non-fuscans) and X. axonopodis pv. vesicatoria strains. Directed AFLP also showed that a high degree of polymorphism is associated with ISXax1 insertion in these strains.
ISXax1是一种属于IS256和Mutator家族的新型插入序列。斑点杂交、Southern杂交和PCR分析表明,ISXax1仅存在于菜豆黄单胞菌菜豆致病变种(fuscans和non-fuscans变种)以及辣椒斑点病菌菌株中。定向AFLP分析还表明,这些菌株中与ISXax1插入相关的多态性程度很高。