Sato Kae, Hosokawa Kazuo, Maeda Mizuo
Bioengineering Laboratory, RIKEN, Wako, Saitama, Japan.
Anal Sci. 2007 Jan;23(1):17-20. doi: 10.2116/analsci.23.17.
In this review, we present an overview of the technologies in colorimetric biosensors based on DNA-nanoparticle conjugates. Two types of DNA-nanoparticles aggregation assays are summarized. One of the methods relies on cross-linking of the gold nanoparticle (GNP) by hybridization. The crosslinking system was used not only to detect target DNA sequences, but also to detect metal ions or small molecules which were recognized by DNAzymes. The other method is the GNP non-crosslinking system. This approach shows high performance in the detection of single nucleotide polymorphisms. These methods do not need special equipment and open up a new possibility of point-of-care diagnoses.
在本综述中，我们概述了基于DNA-纳米颗粒共轭物的比色生物传感器技术。总结了两种类型的DNA-纳米颗粒聚集分析方法。其中一种方法依赖于通过杂交对金纳米颗粒（GNP）进行交联。该交联系统不仅用于检测目标DNA序列，还用于检测被DNA酶识别的金属离子或小分子。另一种方法是GNP非交联系统。这种方法在单核苷酸多态性检测中表现出高性能。这些方法不需要特殊设备，并为即时诊断开辟了新的可能性。
