Tauran Yannick, Brioude Arnaud, Coleman Anthony W, Rhimi Moez, Kim Beonjoom
Yannick Tauran, Arnaud Brioude, Anthony W Coleman, CNRS, LMI, University of Lyon 1, F69622 Villeurbanne, France.
World J Biol Chem. 2013 Aug 26;4(3):35-63. doi: 10.4331/wjbc.v4.i3.35.
The intrinsic physical properties of the noble metal nanoparticles, which are highly sensitive to the nature of their local molecular environment, make such systems ideal for the detection of molecular recognition events. The current review describes the state of the art concerning molecular recognition of Noble metal nanoparticles. In the first part the preparation of such nanoparticles is discussed along with methods of capping and stabilization. A brief discussion of the three common methods of functionalization: Electrostatic adsorption; Chemisorption; Affinity-based coordination is given. In the second section a discussion of the optical and electrical properties of nanoparticles is given to aid the reader in understanding the use of such properties in molecular recognition. In the main section the various types of capping agents for molecular recognition; nucleic acid coatings, protein coatings and molecules from the family of supramolecular chemistry are described along with their numerous applications. Emphasis for the nucleic acids is on complementary oligonucleotide and aptamer recognition. For the proteins the recognition properties of antibodies form the core of the section. With respect to the supramolecular systems the cyclodextrins, calix[n]arenes, dendrimers, crown ethers and the cucurbitales are treated in depth. Finally a short section deals with the possible toxicity of the nanoparticles, a concern in public health.
贵金属纳米颗粒的固有物理性质对其局部分子环境的性质高度敏感，使得此类体系成为检测分子识别事件的理想选择。本综述描述了贵金属纳米颗粒分子识别的现状。第一部分讨论了此类纳米颗粒的制备以及包覆和稳定化方法。简要讨论了三种常见的功能化方法：静电吸附、化学吸附、基于亲和力的配位。第二部分讨论了纳米颗粒的光学和电学性质，以帮助读者理解这些性质在分子识别中的应用。在主要部分，描述了用于分子识别的各种类型的包覆剂；核酸涂层、蛋白质涂层以及超分子化学家族的分子，并介绍了它们的众多应用。核酸方面重点介绍互补寡核苷酸和适体识别。蛋白质方面，抗体的识别特性是该部分的核心。对于超分子体系，深入探讨了环糊精、杯[n]芳烃、树枝状大分子、冠醚和葫芦脲。最后一小节讨论了纳米颗粒可能的毒性，这是公共卫生领域关注的问题。
