Kikuchi-Taura Akie, Soma Toshihiro, Matsuyama Tomohiro, Stern David M, Taguchi Akihiko
Department of Clinical Laboratory, National Hospital Organization, Osaka Minami Medical Center, Osaka 586-8521, Japan.
Tex Heart Inst J. 2006;33(4):427-9.
Increasing evidence points to a role for circulating CD34-positive (CD34(+)) cells in vascular maintenance and neovascularization. Although there are established methods for evaluating absolute numbers of CD34(+) cells in bone marrow or mobilized peripheral blood, there is no convenient and highly reproducible method for quantifying low numbers of CD34(+) cells in blood samples, such as those from the peripheral blood of patients with cardiovascular disease. With current commonly used methods, the mean percentage of CD34(+) cells in leukocyte fractions from such patients was only 0.02%, and the cumulative intra-assay coefficient of variation was approximately 30%. With use of the protocol described herein, actual counts of CD34(+) cells increased approximately 5-fold and cumulative intra-assay coefficients of variation were reduced to approximately 7%. The new method is useful to precisely measure low numbers of CD34(+) cells in samples, and it has potential as a screening tool to evaluate cardiovascular risk in large patient populations.
越来越多的证据表明，循环中的CD34阳性（CD34(+)）细胞在血管维持和新生血管形成中发挥作用。虽然已有评估骨髓或动员外周血中CD34(+)细胞绝对数量的方法，但对于定量血样（如心血管疾病患者外周血中的血样）中少量CD34(+)细胞，尚无便捷且高度可重复的方法。使用当前常用方法，此类患者白细胞组分中CD34(+)细胞的平均百分比仅为0.02%，且检测内累积变异系数约为30%。使用本文所述方案，CD34(+)细胞的实际计数增加了约5倍，检测内累积变异系数降至约7%。该新方法有助于精确测量样本中少量的CD34(+)细胞，并且有潜力作为一种筛查工具，用于评估大量患者群体的心血管风险。
