Navarro V, Miranda J, Churruca I, Fernández-Quintela A, Rodríguez V M, Portillo M P
Department of Nutrition and Food Science, University of Pais Vasco, Paseo de la Universidad 7. 01006 Vitoria, Spain.
J Physiol Biochem. 2006 Jun;62(2):81-7. doi: 10.1007/BF03174069.
The aim of the present work was to determine whether t-10, c-12 conjugated linoleic acid (CLA) feeding was able to reduce body fat accumulation and improve the serum lipid profile in adult hamsters fed an atherogenic diet, in order to compare these effects with those observed in young growing hamsters. Young and adult hamsters were fed semi-purified atherogenic diets supplemented with 0.5 % linoleic acid or 0.5% t-10, c-12 CLA for 6 weeks. Body weight and food intake were measured every two days. Adipose tissue from different anatomical locations, liver and gastrocnemious muscle were dissected and weighed. Cholesterol, triacylglycerols, non-esterified fatty acids and proteins were determined spectrophotometrically and water content by gravimetry. In young hamsters, no significant differences were found in food intake, final body weight and gastrocnemious muscle weight. White adipose tissue weights were reduced, liver weight was increased and cholesterol and triacyl-glycerols in both serum and liver were reduced. In adult hamsters, CLA feeding decreased food intake and adipose tissue weights. No changes were observed in other parameters. The present study demonstrates that age has an influence in hamster responsiveness to t-10, c-12 CLA because, although when this isomer is added to an atherogenic diet it reduces body fat accumulation in both young and adults hamsters, the lessening of the effects on serum lipids brought about by atherogenic feeding is only observed in young animals. Moreover, it is clear that liver is a target for CLA in young but not in adult hamsters.
本研究的目的是确定在喂食致动脉粥样化饮食的成年仓鼠中，添加反式-10，顺式-12共轭亚油酸（CLA）是否能够减少体脂积累并改善血脂状况，以便将这些效果与在幼年生长仓鼠中观察到的效果进行比较。幼年和成年仓鼠被喂食添加了0.5%亚油酸或0.5%反式-10，顺式-12 CLA的半纯化致动脉粥样化饮食，持续6周。每两天测量一次体重和食物摄入量。解剖并称重来自不同解剖位置的脂肪组织、肝脏和腓肠肌。采用分光光度法测定胆固醇、三酰甘油、非酯化脂肪酸和蛋白质，并通过重量法测定水分含量。在幼年仓鼠中，食物摄入量、最终体重和腓肠肌重量未发现显著差异。白色脂肪组织重量减少，肝脏重量增加，血清和肝脏中的胆固醇及三酰甘油均减少。在成年仓鼠中，喂食CLA可减少食物摄入量和脂肪组织重量。其他参数未观察到变化。本研究表明，年龄对仓鼠对反式-10，顺式-12 CLA的反应有影响，因为尽管将这种异构体添加到致动脉粥样化饮食中时，它能减少幼年和成年仓鼠的体脂积累，但致动脉粥样化饮食对血脂影响的减轻仅在幼年动物中观察到。此外，很明显肝脏是CLA在幼年仓鼠而非成年仓鼠中的作用靶点。
