Lasa A, Simón E, Churruca I, Fernández-Quintela A, Rodríguez V M, Portillo M P
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain.
J Physiol Biochem. 2007 Dec;63(4):297-304. doi: 10.1007/BF03165761.
Numerous studies have demonstrated that conjugated linoleic acid (CLA) modulates body composition, reducing body fat accumulation in various mammalian species. However, very few studies have been carried out to assess the effect of CLA on previously stored body fat. The aim of the present work was to analyse the effectiveness of trans-10,cis-12 CLA in improving alterations produced by high-fat feeding in body fat and serum parameters when it was included in an energy-restricted diet. For this purpose male Syrian Golden hamsters were fed on high-fat diet for 7 weeks in order to increase their body fat content, and a further 25% energy-restricted diet supplemented or not with 0.5% trans-10,cis-12 CLA for 3 weeks. Adipose tissues, liver and gastrocnemious muscles were dissected and weighed. Adipocyte diameter and number were assessed in epididymal adipose tissue. Total cholesterol, triacylglycerols, non-esterified fatty acids and glucose were measured in serum. Three weeks of energy restriction resulted in a reduction in body weight and white adipose tissue size in all anatomical locations, without changes in liver and gastrocnemious muscle weights. Epididymal adipocyte size was reduced, but total adipocyte number remained unchanged. Serum cholesterol, triacylglycerols and glucose were significantly reduced. No differences were observed between the restricted groups (control and CLA supplemented). In conclusion, under our experimental conditions, the addition of trans-10,cis-12 CLA to the diet does not increase the benefits produced by energy restriction.
众多研究表明，共轭亚油酸（CLA）可调节身体成分，减少各种哺乳动物体内脂肪的积累。然而，很少有研究评估CLA对先前储存的体内脂肪的影响。本研究的目的是分析反式-10，顺式-12 CLA在能量限制饮食中对高脂喂养引起的体脂和血清参数变化的改善效果。为此，雄性叙利亚金仓鼠先高脂饮食7周以增加其体脂含量，然后进一步给予25%能量限制饮食，其中一组补充0.5%反式-1,顺式-12 CLA，另一组不补充，持续3周。解剖并称重脂肪组织、肝脏和腓肠肌。评估附睾脂肪组织中的脂肪细胞直径和数量。测量血清中的总胆固醇、三酰甘油、非酯化脂肪酸和葡萄糖。三周的能量限制导致所有解剖部位的体重和白色脂肪组织大小减少，肝脏和腓肠肌重量无变化。附睾脂肪细胞大小减小，但脂肪细胞总数不变。血清胆固醇、三酰甘油和葡萄糖显著降低。在限制组（对照组和补充CLA组）之间未观察到差异。总之，在我们的实验条件下，在饮食中添加反式-10，顺式-12 CLA不会增加能量限制带来的益处。
