Koh T, Nakazawa M, Kani K, Maeda T
Department of Ophthalmology, Shiga University of Medical Science, Japan.
Brain Res Bull. 1991 Nov;27(5):675-84. doi: 10.1016/0361-9230(91)90044-k.
The present study investigated whether the raphe neurons which give rise to the transient serotonergic fibers in the visual cortex of neonatal rats persist or disappear as the rats mature. Three experiments were performed employing the WGA-apoHRP-Au retrograde transport technique in conjunction with 5-HT or WGA-HRP immunohistochemical staining. WGA-apoHRP-Au was injected into the primary visual cortex of all rats 9 days postnatally. In the first experiment, the animals were examined after 2 days; retrogradely labeled cells were observed in the dorsal raphe nucleus (DR), the median raphe nucleus (MR), and in the B9 and B6 cell groups; the majority (82.5%) of the cells was serotonergic. In the second experiment, the examinations took place following a survival time of 8 weeks: virtually all of the original raphe-visual cortical serotonergic neurons were found to the present. In the third experiment, also performed after 8 weeks relabeling the raphe-visual cortical neurons by WGA-HRP, it was found that 37.2% of the raphe neurons which had projected to the neonatal visual cortex no longer possessed such projections.
本研究调查了新生大鼠视觉皮层中产生短暂性血清素能纤维的中缝神经元在大鼠成熟过程中是持续存在还是消失。采用WGA-apoHRP-Au逆行运输技术结合5-HT或WGA-HRP免疫组织化学染色进行了三个实验。在出生后第9天，将WGA-apoHRP-Au注射到所有大鼠的初级视觉皮层。在第一个实验中，2天后检查动物；在中缝背核（DR）、中缝正中核（MR）以及B9和B6细胞群中观察到逆行标记的细胞；大多数（82.5%）细胞是血清素能的。在第二个实验中，在存活8周后进行检查：发现几乎所有原来的中缝-视觉皮层血清素能神经元都存在。在第三个实验中，同样在8周后通过WGA-HRP对中缝-视觉皮层神经元进行重新标记，发现投射到新生大鼠视觉皮层的中缝神经元中有37.2%不再有这样的投射。
