Xiao Chao-Ting, Zhang Ming-Hai, Fu Yan, Koh Hung-Son
College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
Biochem Genet. 2007 Feb;45(1-2):93-102. doi: 10.1007/s10528-006-9052-z.
Partial sequences of the mitochondrial control region of northeastern China roe deer were analyzed to determine the degree of genetic diversity. Fourteen haplotypes were observed. The haplotype diversity was high (h = 0.872), nucleotide diversity was medium (p ( i ) = 0.0108), and the average Tamura-Nei nucleotide distance among them was 1.9%, indicating that genetic diversity of roe deer from northeastern China was relatively high and that the effective population size was large historically. To clarify the northeastern China roe deer's taxonomic status, these 14 haplotypes were compared with 31 haplotypes published in Genbank from Europe, Siberia, and Korea. The average genetic distance between haplogroups of northeastern China and European roe deer (5.8%) was more than twice that between northeastern China and Siberian roe deer (2.7%), indicating sufficient variation to consider roe deer of northeastern China and Siberia as a single species (Capreolus pygargus), distinct from European roe deer (Capreolus capreolus). This is the first presentation of mtDNA data for roe deer in northeastern China, which will be helpful in investigations of genetic diversity and clarifications of the taxonomic status of roe deer in the whole of China.
对中国东北狍线粒体控制区的部分序列进行分析，以确定其遗传多样性程度。共观察到14种单倍型。单倍型多样性较高（h = 0.872），核苷酸多样性中等（p(i)= 0.0108），它们之间的平均Tamura-Nei核苷酸距离为1.9%，表明中国东北狍的遗传多样性相对较高，历史上有效种群数量较大。为了阐明中国东北狍的分类地位，将这14种单倍型与Genbank中公布的来自欧洲、西伯利亚和韩国的31种单倍型进行了比较。中国东北狍与欧洲狍单倍型组之间的平均遗传距离（5.8%）是中国东北狍与西伯利亚狍之间平均遗传距离（2.7%）的两倍多，这表明有足够的差异可将中国东北狍和西伯利亚狍视为一个物种（狍Capreolus pygargus），与欧洲狍（Capreolus capreolus）不同。这是首次展示中国东北狍的线粒体DNA数据，这将有助于对中国狍的遗传多样性进行研究，并阐明其分类地位。
