Lee Yun Sun, Markov Nickolay, Argunov Alexander, Voloshina Inna, Bayarlkhagva Damdingiin, Kim Baek-Jun, Min Mi-Sook, Lee Hang, Kim Kyung Seok
Conservation Genome Resource Bank for Korean Wildlife College of Veterinary Medicine and Research Institute for Veterinary Science Seoul National University Seoul Korea.
Institute of Plant and Animal Ecology Urals Branch of Russian Academy of Sciences Yekaterinburg Russia.
Ecol Evol. 2016 Sep 22;6(20):7286-7297. doi: 10.1002/ece3.2458. eCollection 2016 Oct.
Current understanding of phylogeographical structure and genetic diversity of Siberian roe deer remains limited mainly due to small sample size and/or low geographical coverage in previous studies. Published data suggest at least two phylogroups: western (Ural Mountains and Western Siberia) and eastern (east from lake Baikal, including the Korean peninsula), but their phylogenetic relationship remains unclear. Combined sequences of cytochrome (1140 bp) and the mtDNA control region (963 bp) were analyzed from 219 Siberian roe deer from 12 locations in Russia, Mongolia, and South Korea, which cover a large part of its range, to assess genetic diversity and phylogeographical status. Special emphasis was placed on the demographic history and genetic features of central, peripheral, and isolated populations. Results of median-joining network and phylogenetic tree analyses indicate that Siberian roe deer from the Urals to the Pacific Ocean are genetically diverse and that geographical distribution and composition of haplogroups coincide with previously described ranges of the subspecies and . We found that peripheral populations in the northwestern parts of the species range (Urals), as well as the isolated population from Jeju Island, are genetically distinct from those in the core part of the range, both in terms of genetic diversity and quantitative composition of haplogroups. We also found that northwestern (Urals) and northern (Yakutia) peripheral populations share the same haplogroup and fall into the same phylogenetic clade with the isolated population from Jeju Island. This finding sheds light on the taxonomic status of the Jeju Island population and leads to hypotheses about the discordance of morphological and genetic evolution in isolated populations and specific genetic features of peripheral populations.
目前，对于西伯利亚狍的系统地理学结构和遗传多样性的理解仍然有限，这主要是由于先前研究中的样本量较小和/或地理覆盖范围较窄。已发表的数据表明至少存在两个系统群：西部（乌拉尔山脉和西西伯利亚）和东部（贝加尔湖以东，包括朝鲜半岛），但它们的系统发育关系仍不清楚。对来自俄罗斯、蒙古和韩国12个地点的219只西伯利亚狍的细胞色素b（1140bp）和线粒体DNA控制区（963bp）的组合序列进行了分析，这些地点覆盖了其分布范围的很大一部分，以评估遗传多样性和系统地理状况。特别强调了中部、边缘和孤立种群的种群历史和遗传特征。中介连接网络和系统发育树分析结果表明，从乌拉尔山脉到太平洋的西伯利亚狍在遗传上具有多样性，单倍群的地理分布和组成与先前描述的亚种和的范围一致。我们发现，该物种分布范围西北部（乌拉尔山脉）的边缘种群以及济州岛的孤立种群，在遗传多样性和单倍群的数量组成方面，在遗传上都与分布范围核心部分的种群不同。我们还发现，西北部（乌拉尔山脉）和北部（雅库特）的边缘种群与济州岛的孤立种群共享相同的单倍群，并属于同一系统发育分支。这一发现揭示了济州岛种群的分类地位，并引发了关于孤立种群形态和遗传进化不一致以及边缘种群特定遗传特征的假设。
