Friedlaender G E, Mitchell M S
Cancer. 1975 Nov;36(5):1631-9. doi: 10.1002/1097-0142(197511)36:5<1631::aid-cncr2820360516>3.0.co;2-w.
Inoculation of Moloney sarcoma virus into the marrow cavity of the tibia of newborn Wistar-Lewis rats resulted in the appearance of an initially localized osteosarcoma in 97.7% of these animals. At least 77.9% of the rats developed lung metastases and died, usually within 6 weeks of inoculation. The remaining 22.1% showed regression of disease after initial growth of the tumor. Tumor cells were maintained in tissue culture and used as target cells for a visual and isotopic (3H-thymidine or 125IUdR) microcytotoxicity assay. Cell-mediated immunity could be measured by these methods throughout the course of the illness in animals with progressive disease as well as in those whose tumors eventually regressed. The presence of serum factors capable of modifying the level of CMI was documented. This Moloney-sarcoma-virus-induced rat osteosarcoma and human osteosarcoma thus appear to have several basic pathologic and immunologic similarities. The model may be useful for studying the effects of a variety of treatment protocols upon the clinical course and immune response to osteosarcoma.
将莫洛尼肉瘤病毒接种到新生Wistar-Lewis大鼠的胫骨髓腔内，97.7%的动物出现了最初局限的骨肉瘤。至少77.9%的大鼠发生肺转移并死亡，通常在接种后6周内死亡。其余22.1%的大鼠在肿瘤最初生长后疾病出现消退。肿瘤细胞在组织培养中保存，并用作视觉和同位素（3H-胸腺嘧啶核苷或125I-尿苷）微细胞毒性测定的靶细胞。在疾病进展的动物以及肿瘤最终消退的动物整个病程中，均可通过这些方法测量细胞介导的免疫。已证明存在能够改变细胞介导免疫水平的血清因子。因此，这种莫洛尼肉瘤病毒诱导的大鼠骨肉瘤和人类骨肉瘤似乎具有若干基本的病理和免疫学相似性。该模型可能有助于研究各种治疗方案对骨肉瘤临床病程和免疫反应的影响。
